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KMID : 0914820030030010019
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Journal of the Korean Gastric Cancer Association
2003 Volume.3 No. 1 p.19 ~ p.25
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Expression of Survivin and HIAP-1 in Korean Gastric Cancers
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Park Chan-Jin
Ryu Seung-Wan
Kim In-Ho
Baek Won-Ki
Suh Seong-Il
Suh Min-Ho
Sohn Soo-Sang
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Abstract
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Purpose: Dysregulation of apoptosis may attribute to development of cancer by abnormally prolonging cell viability with accumulation of transforming mutations. Survivin and HIAP (Human Inhibitors of Apoptosis)-1 were recently described as apoptosis inhibitors. Their pathogenic roles in gastric cancer are largely unknown. In the present study, we examined the expression of survivin and HIAP-1 in gastric cancer tissues and cell lines in order to elucidate the roles of survivin and HIAP-1 in the process of gastric carcinogenesis.
Materials and Methods: Eight gastric cancer cell lines and five gastric cancer tissues were studied. The expression of survivin and HIAP-1 were evaluated by reverse transcription -polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot.
Results: Western blot and RT-PCR analysis revealed survivin and HIAP-1 expression in all gastric cancer cell lines. Increased expression of survivin and HIAP-1 were found in all cases of gastric cancer tissues compared to normal tissues by Western blot analysis. In immunohistochemical analysis tumor cells were stained with anti-survivin and anti-HIAP-1 antibodies. Cell cycle dependence of survivin expression was preserved in gastric cancer cell lines.
Conclusion: The results indicate that increased expression of survivin and HIAP-1 genes may play an important role in gastric cancer.
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KEYWORD
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Gastric cancer, Survivin, HIAP-1
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